Journal: Pain

Article Title: Mast cell corticotropin-releasing factor receptor 1 contributes to pancreatic cancer pain via mitogen-activated protein kinase/sphingosine kinases type 1 signaling

doi: 10.1097/j.pain.0000000000003909

Figure Lengend Snippet: MAPK/SPHK1 axis is a potential downstream pathway of CRFR1 in mast cell activation. (A) Volcano plot showed 134 upregulated and 394 downregulated genes in para-tumor tissues from CRFR1-CKO mice compared to WT controls. (B) Heatmap of the top 100 differentially expressed genes. (C) GO enrichment analysis revealed key altered pathways, including MAPK signaling. (D) GSEA indicated that CRFR1 knockout suppressed the phospholipid biosynthetic process. (E and F) qRT-PCR and western blotting confirmed the downregulation of SPHK1 after CRFR1 inhibition. (G) ELISA results showing reduced S1P levels in CRFR1-CKO mice. (H) CRF stimulation increased SPHK1 expression and activated MAPK signaling in MC/9 mast cells in vitro. (I) CRFR1 knockout reduced MAPK pathway activation in vivo. (J) MAPK inhibitor SB203580 blocked CRF-induced SPHK1 upregulation. Data with error bars represented as mean ± SD and analyzed using 2-tailed Student t test. * P < 0.05, ** P < 0.01, *** P < 0.001. CKO, conditional knockout; CRF, corticotropin-releasing factor; CRFR1, corticotropin-releasing factor receptor 1; ELISA, enzyme-linked immunosorbent assay; ERK, extracellular signal-regulated kinase; GO, gene ontology; GSEA, gene set enrichment analysis; MAPK, mitogen-activated protein kinase; PC, orthotopic pancreatic cancer model mice; qRT-PCR, quantitative real-time polymerase chain reaction; S1P, sphingosine-1-phosphate; SPHK1, sphingosine kinases type 1; WT, wild type.

Article Snippet: The murine PDAC cell line Panc-02 and mast cell line MC/9 were obtained from Servicebio Biotechnology Co., Ltd. (Wuhan, China).

Techniques: Activation Assay, Knock-Out, Quantitative RT-PCR, Western Blot, Inhibition, Enzyme-linked Immunosorbent Assay, Expressing, In Vitro, In Vivo, Real-time Polymerase Chain Reaction

Journal: Translational Gastroenterology and Hepatology

Article Title: Eosinophilic and mastocytic gastrointestinal diseases: current knowledge and controversy in histopathologic diagnosis

doi: 10.21037/tgh-2025-146

Figure Lengend Snippet: Mastocytosis involving the colon. (A) Histology showing lamina propria aggregates of mast cells, some having a spindled morphology. Note the presence of prominent eosinophils (hematoxylin-eosin stain, original magnification ×400). (B) Immunostaining for CD117 highlighting sheets of mast cells in the lamina propria (original magnification ×200). (C) Mast cells co-expressing CD25 (immunohistochemistry, original magnification ×200).

Article Snippet: There are three potential types of mast cell involvement of the GI tract: abnormal mucosal mast cell aggregates (mastocytosis), so-called ME, and MCAS.

Techniques: Staining, Immunostaining, Expressing, Immunohistochemistry